Analysis of lycopene in tomato (Lycopersicon esculentum, Mill.) fruits
für 44.10€ kaufen ··· 9783847327486 ··· 10361100102 ··· Lycopene is highly unsaturated, open-chain carotenoid found abundantly in tomatoes. Research interests into properties & health benefits of lycopene have been growing, bringing attention to content of lycopene in food. The purpose of this work was to develop efficient & accurate protocol that can measure lycopene using HPLC, and establish relationship with Vis/NIR reflectance spectroscopy measurement. Accordingly, mobile phase composition ACN/MeOH (50:50, v/v)+ TEA 9 µM extraction solvents hexane/acetone/ethanol (50:25:25, v/v/v) re-dissolving residue in THF, followed ACN/MeOH (15:30:55, v/v/v) flow rate 0.6 mL/min and detection 472 nm were showed most suitable for lycopene determination in tomato. This method has achieved best characteristic spectra profile with precision (RSD 15), accuracy & recovery ( 81.7%) and sensitive detection limit (0.0156 g/mL) within separation of ~ 21 minutes. Finally, the established method was applied to selected tomato cultivars and a wide range of lycopene content 1.91 to 8.23 mg/100g was observed. Besides, although HPLC is most accepted method Vis/NIR combined with multivariate techniques has showed potential for determining lycopene. Hersteller: LAP Lambert Academic Publishing Marke: LAP Lambert Academic Publishing EAN: 9783847327486 Kat: Hardcover/Naturwissenschaften, Medizin, Informatik, Technik/Biologie/Landwirtschaft, Gartenbau, Forstwirtschaft, Fischerei, Ernährung Lieferzeit: Sofort lieferbar Versandkosten: Ab 20¤ Versandkostenfrei in Deutschland Icon: https://www.inforius-bilder.de/bild/?I=b2MWhlfbXAvKbgiew3fJQ18aiDxr%2FocovMi1mLwfNsQ%3D Bild: