Enhancement of xylitol production in Candida tropicalis
für 44.10€ kaufen ··· 9783847335016 ··· 10361182 ··· In this study, gk gene was disrupted from xylitol dehydrogenase gene (XYL2) knockout C. tropicalis strain BSXDH-3. The resulting strain was incapable to grow on glycerol. The cells growth on glycerol was resumed by co-expression of gcy1, 2 and 3 genes from Scheffersomyces stipitis under the control of C. tropicalis glyceraldehydes-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed IA-3 , than in BSXDH-3. The pentose phosphate pathway (PPP) is a major basis of NADPH biosynthesis in C. tropicalis. In order to increase xylitol production rate, xylitol dehydrogenase gene (XYL2)-disrupted C. tropicalis strain BSXDH-3 was engineered to co-express zwf and gnd genes, which respectively encode glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6-PGDH), under the control of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) promoter. NADPH-dependent xylitol production was higher in the engineered strain, termed `PP`, than in BSXDH-3. This is the first report of increased metabolic flux toward glycerol metabolic and PPP in C. tropicalis for NADPH regeneration and enhanced xylitol production. Hersteller: LAP Lambert Academic Publishing Marke: LAP Lambert Academic Publishing EAN: 9783847335016 Kat: Hardcover/Naturwissenschaften, Medizin, Informatik, Technik/Naturwissenschaften allgemein Lieferzeit: Sofort lieferbar Versandkosten: Ab 20¤ Versandkostenfrei in Deutschland Icon: https://www.inforius-bilder.de/bild/?I=Z7jxah60i7UmcBRnHeRUeFgIDctIEtGYbbVWUO%2Bm0c8%3D Bild:
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