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Plastic monomer degradation - Engineering Pseudomonas putida KT2440 for plastic monomer utilization

····· lezzter Preis 38.27€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
The environmental impact of plastic waste is regularly discussed. With bio-upcycling we can use plastic waste as a substrate for biotechnology. Polymers like PET and PU can be hydrolyzed to yield monomers like adipic acid, 1,4-butandiol and ethylene glycol. This work enabled Pseudomonas putida KT2440 to utilize these monomers, and gave insights into their metabolism and its regulation. Hereby, a biological basis to produce value added materials from plastic monomers was set. ····· 1036178950

High Quality 13C metabolic flux analysis using GC-MS

····· lezzter Preis 38.27€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
To reduce the demand for fossil resources, biotechnological processes are increasingly applied. Hence, the used biological hosts have to be optimized in order to achieve the highest possible yield. Prior to this optimization, which is usually carried out by means of metabolic engineering strategies, the intracellular processes need to be investigated by methods such as 13C-MFA. The present thesis focussed on improving the labelling determination analysis for 13C-MFA based on GC-MS and GC-MS/MS. ····· 1036178763

Accessing natural diversity of rhamnolipids by metabolic engineering of Pseudomonas putida

····· lezzter Preis 38.27€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
The petrochemical industry is essential for many consumer goods currently in use. To implement innovative biobased processes, renewable resources are used as substitutes for crude oil in goods such as cleaning agents. However, biosurfactants are still expensive compared to their chemically synthesized counterparts. In this work, the exploitation of Pseudomonas putida for the heterologous synthesis of rhamnolipids, glycolipids with a high potential for industrial applications was the main focus. ····· 1036178562

Mikrobiologische Kontamination von Heizöl - Ursachen und Auswirkungen auf Brennstoff und Tank

····· lezzter Preis 38.27€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Ziel des vorliegenden Forschungsprojektes war es, nach vermehrten Kontaminationen im Dieselsektor, Faktoren aufzudecken, welche potentielle Kontamination des chemisch ähnlichen Heizöls verursachen und fördern. Dabei wurden die Auswirkungen einer Kontamination auf die in Heizölbrennersystemen eingesetzten Materialien und Bauteile untersucht und die an der Kontamination beteiligten Mikroorganismen identifiziert. In allen Experimenten wurde der Einfluss der zur Beimischung in Heizöl geplanten Biokomponente FAME (Fettsäuremethylester, engl.: Fatty Acid Methyl Ester) untersucht. ····· 1036178502

Itaconic Acid Production by Ustilago maydis

····· lezzter Preis 38.27€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
This thesis endorses the establishment of U. maydis as an alternative whole cell biocatalyst for the biosynthesis of itaconate from renewable substrates, such as cellulose or hemicellulose. Therefore, the fungal family Ustilaginaceae was screened for their natural production of value added chemicals, such as itaconate, malate, or succinate, to identify potential novel natural biocatalysts and to find the best producers. The screening was facilitated by the development of a new medium composition (modified Tabuchi medium). In order to combine the utilization of raw biomass components, such as cellulose and hemicellulose, with the production of valuable platform chemicals, such as itaconate, the best itaconate producer, U. maydis MB215, was further characterized towards its xylan degradation ability and itaconate production. A novel biosynthesis pathway for itaconate was identified, including clustered genes encoding the proteins responsible for itaconate production in U. maydis MB215. First metabolic engineering attempts were already successful in enhancing U. maydis` itaconate production two-fold by overexpressing the transcription factor regulating the expression of the itaconate gene cluster. This work lays the foundation for further optimization of U. maydis` itaconate biosynthesis and is therefore a further step towards industrial application of the Ustilaginaceae for the advancement of a sustainable bio-based economy. ····· 1036178449

EM-Lösungen - Ernährung

····· lezzter Preis 14.95€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Wer auch im fortgeschrittenen Lebensalter gesund, munter und leistungsfähig bleiben möchte, muss beizeiten vorsorgen. Dieses Buch zeigt Ihnen, wie Sie mit der richtigen Ernährung dafür die Grundlage schaffen und erklärt die Zusammenhänge. ····· 1036171772

EM Lösungen Teiche, Zier- und Fischteiche, Schwimmteiche, Pools

····· lezzter Preis 9.95€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Ein Gartenteich ist eine Zierde für den Garten und eine Freude für den Besitzer - vorausgesetzt das Wasser ist klar und die Pflanzen sind gesund. Wie dies mithilfe von Effektiven Mikroorganismen (EM) auf natürliche Weise erreicht werden kann, wird in diesem Buch ausführlich geschildert. ····· 1036171768

Organische Düngung und reduzierte Bodenbearbeitung als Steuerungsfaktoren für die C-, N-, P- und S-Speicherung von Mikroorganismen

····· lezzter Preis 29.00€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Vor allem in intensiv genutzten Systemen, wie der Landwirtschaft, wird dem Kompartiment Boden und seiner nachhaltigen Nutzen immer größere Aufmerksamkeit geschenkt. Die vorliegende Studie untersucht die Faktoren Düngung und Bodenbearbeitung als wichtige Steuerungselement von Bodenmanagement. ····· 1036162217

Group C and group G Streptococci

····· lezzter Preis 44.10€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
The Streptococcus milleri group (SMG) is a highly diverse group which includes three species: Streptococcus anginosus, Streptococcus intermedius, and Streptococcus constellatus. The group also includes hemolytic streptococci belonging to Lancefield group A, C, F or G as well as non-groupable and non-hemolytic streptococci. The nomenclature, identification and classification of Streptococcus milleri have been confusing. In 1989, it was proposed in the United States to rename this different group under one species name, Streptococcus anginosus, while in Great Britain the designation Strep. milleri was preferred. SMG is commensal organisms commonly isolated from mouth, oropharynx, gastrointestinal tract and vagina, but they can cause a variety of human and animal infections. The roles of group C streptococci in causing endemic pharyngitis are still controversial. This book provides data concerning phenotypic characterization of group C and G streptococci in patients with acute pharyngitis, their virulence and antibiotic resistance, and their role in causing pharyngitis among other groups of Streptococci. ····· 1036125986

Comparison Between MGIT 960 and Bactec 460 TB system

····· lezzter Preis 44.10€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
This study was carried out at the department of Microbiology, Armed Forces Institute of Pathology, Rawalpindi, Pakistan. The purpose of this study was to find out the recovery rate of mycobacteria on MGIT 960 system and to compare it with the already in use Bactec 460 TB system. The study was carried out on various human clinical specimens submitted in our institute for culture of mycobacteria. All the specimens were processed as per the recommended protocol and inoculated into both MGIT 960 and Bactec 460 TB system. Our results showed that time to detection of mycoacteria on MGIT 960 was earlier as compared to Bactec 460 system while contamination rate on MGIT 960 system was more. In conclusion, the fully automated, rapid MGIT 960 system is better than the semi-automated Bactec 460 TB system and this system has been used as Gold standard in many parts of the world. ····· 1036125964

Isolation of Pyrethroid Degrading Bacteria From Rhizosphere of Plants

····· lezzter Preis 44.10€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Pesticide usage has become an essential component of modern age agriculture. Their applications for the protection of crops through the pests, insects, mites, ticks and weeds are increasing steadily. The Pyrethroids constitute about twenty five percent of total pesticides used worldwide. These are based on an ester, derived from a flower chrysanthemum. Bifenthrin and cypermethrin are amongst natural Pyrethrins used. The excessive use of pesticides creates different environmental problems including soil and water pollution and ecological imbalance. Soil microflora the hidden creatures in earth`s biodiversity and the soil microbial community are physiologically versatile in metabolizing and mineralizing a wide variety of organic pollutants into non toxic form. The utilization of microorganisms to clean up xenobiotics from polluted environment present a cheap and biologically safe solution for pollution problem. The present work deals with isolation and chracterization of pyrethroid degrading bacteria from rhizospheric soil of tannery solid waste. Chromatographic separation of degraded products show the capacity of bacteria to be used as a biological tool for bioremediation. ····· 1036125913

Antimicrobial Studies on Conventional & Cultured Plant Tissue Extracts

····· lezzter Preis 53.10€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Plants have been the source of treatment for many ailments of man since times immemorial. With progress in man s intelligence many indigenous medicinal systems based on herbal drugs, obtained from a wide variety of plants possessing therapeutic properties, developed all over the world. These are in use even today. Most of these natural products or drugs are derived from secondary metabolites of plants. Generally, production of secondary metabolites is high in plant tissues developed in vitro rather than the conventionally grown plant tissues. The secondary metabolites serve as plant defence mechanisms against microorganisms, insects and herbivores. In the present study, thirty different indigenous traditional medicinal plants were selected for an analysis of their antimicrobial activity along with the in vitro cultured tissues of the same plants, against some pathogenic microorganisms and the results were compared. The experimental plants solvent extracts were also subjected to preliminary phytochemical screening so as to identify the chemical constituents (bioactive components) which may be responsible for the antimicrobial activity exhibited. ····· 1036125834

Mixed Yeast Infections

····· lezzter Preis 53.10€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Different yeast species may exist singly or in combination with each other,they infect human, different animal species as well as poultry. In Qena Governorate (Upper Egypt), some of these isolates were identified with the ordinary mycological methods,biochemical assays and molecular biological techniques. Samples were collected from both diseased and apparently healthy different animal species and chickens. The number of samples examined mycologically was 4527 including faecal samples, rectal swabs, nasal swabs, vaginal swabs, ear swabs, milk samples, conjunctival swabs, blood samples, chicken viscera and cloacal swabs. They yielded 535 positive samples for different yeast species in a percentage of (11.81%). ····· 1036125810

Impact of Herbals in Wound Infection

····· lezzter Preis 39.90€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Wound infections caused by the bacteria and fungi leads to complications in the infected area which increases disease incidence and morbidity. Present study involves isolation of pathogens from post operative and diabetic wounds. Three herbal plants with therapeutic potential were used to prepare extract in different solvents such as ethanol, acetone and water to cure wound infections. These solvent extracts show considerable inhibitory activity against the isolated wound infection caused by the pathogens. Maximum use of natural medicine from herbals can be a remedial measure to avoid drug resistance in pathogens causing wound infection. ····· 1036125697

Microbiologically Influenced Corrosion of Buried Mild Steel Pipes

····· lezzter Preis 61.20€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Within the biosphere every object is influenced by microbial activities. A very unfamiliar case is represented by the microorganisms involved in the process of corrosion of different metals and their alloys. Many workers have reported bacteria responsible for initiating, accelariting and promoting the process of corrosion. While several recent authors have also documented corrosion inhibiting/controlling roles of microorganisms. It appears that like a healthier balance between the normal microbiota and pathogens, required for maintaining health of a host a comparable balance among the members of microbial community of soil is required for protecting the buried mild steel pipes from corrosion based losses. This work reports isolation of both corrosion enhancing as well as corrosion controlling bacteria from an identified location. The bacterial isolates were then studied under laboratory but field simulated conditions to explore the roles of the monocultures of both catogaries of the bacteria. While effects of their co-cultures have also been delineated in comparable experimental set up. The findings are helpful in controlling corrosion of soil buried mild steel pipes. ····· 1036125644

Bioremediation of Sewage Water and its Impact on Plant Growth

····· lezzter Preis 61.20€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Bioremediation is a pollution control technology that uses biological system to catalyze the degradation of various toxic forms to less harmful forms. Bioremediation could be employed for the treatment of various effluents. The microbial population has an extensive capacity to degrade a variety of organic compounds. In present study, bioremediation of waste water using effective bacterial consortium, different bacterial cultures were isolated from different water samples, in which some effective bacterial cultures possessed efficiency to reduce the physico-chemical parameters of waste water. The bacterial consortium showed more reduction in the parameters in comparison to single bacterial culture. The strategy of bacterial consortium will also contribute to waste water mineralization efficiency. The findings of this study also indicate that the low concentration of untreated waste water showed the stimulatory effect on seedling growth, plant growth & biochemical analysis of Cicer arietinum, whereas the higher concentration of bacteria consortium treated sewage water sample showed more stimulatory effect. ····· 1036125636

Potential of Herbal Preparations as Biopreservatives

····· lezzter Preis 61.20€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
The increase of food borne infections has initiated considerable research interest towards the discovery of potent antimicrobial agents. However, the recent negative consumer perception against artificial preservatives, has shifted the research effort towards the development of alternatives that consumers perceive as naturals. In this era of increased concern on food safety, natural methods of preservation are receiving increased attention. Biopreservatives- a wide range of natural products from both plants and microorganisms- can be useful in extending shelf life of foods, reducing or eliminating pathogenic bacteria and increasing overall quality of food products. The present study confirms that essential oils (clove, cinnamon and peppermint) and herbal extracts (amchur, tamarind, clove, cinnamon and amla) possess in vitro antibacterial and antifungal activity and they can be used as biopreservatives in various food stuffs. Replacement or combination of chemical additives with spices could attenuate the levels of consumption of these chemicals. ····· 1036125557

Tannase-Producing Fungi

····· lezzter Preis 61.20€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Enzymes are highly efficient environment-friendly catalysts, synthesized by living systems. They have significant advantages over chemical catalysts, of which the most important are specificity, high catalytic activity, ability to work at moderate temperatures, and the ability to be produced in large amounts. Enzymes production is a major and growing field of contemporary biotechnology. Tannin acyl hydrolase (EC 3.1.1.20) which is commonly referred to as tannase is one of the important hydrolytic microbial enzymes. Tannase is an industrially important enzyme and has several applications in various industries such as foods, animal feeds, cosmetics, pharmaceutical, chemical, leather industries etc. Realizing the importance of this enzyme, a comprehensive study focusing on its microbial production has been carried out. It aims to the isolation and screening of high tannase-producing fungi from environmental sources and statistical optimization of its production by the promising isolate using response surface methodology. This book includes all details of this study and its important results. ····· 1036125401

Comparative treatment of well and stream water for drinking

····· lezzter Preis 44.10€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
The objective of this research work was to compare the coagulating power of Moringa oleifera seeds with that of alum.Raw water samples were collected from various wells and streams in Modakeke area,Ile-Ife,Osun state,Nigeria.Results show that Moringa oleifera seed is a better coagulating agent than alum because it had an average coagulating power of 85.3% while that of alum was 78.3%.The filter bed had 85.7% reduction in microbial load for Moringa oleifera treated water while there was 79.1% reduction in microbial load for alum treated water.Also,the effectiveness of solar disinfection for the Moringa oleifera treated clear filtrate was 98.1% while that of alum treated water was 91.5%.Hence,Moringa oleifera,sand filtration and solar energy are good alternatives for water treatment especially in rural areas that can`t afford sophisticated water treatment plant or chemicals. ····· 1036125371

Antibacterial-resistance of Klebsiella

····· lezzter Preis 61.20€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Incidence of Antibacterial Resistant Klebsiella in some parts of South Western Nigeria was conceived out of the desire to trace the causes of antibiotics resistance which is now threatening empirical treatments in clinical enviroments. Klebsiella have long been a source of worries in the cause of urinary tract infection and a major source of public health concern. In the paediatric wards, nosocomial Klebsiella infections are especially troublesome particularly in infants and also in intensive care units. Klebsiella are often the pathogens involved in neonatal sepsis, in both early-manisfetation and late manisfestation infections. From the relative distribution of Klebsiella species across Nigeria, highest resistance came from tetracycline followed by augmentin antibiotics, while the least came from ofloxacin. In the case of third generation cephalosporine antibiotics, resistance was highest with ceftazidime and lowest with ceftriaxone. Lagos State had the highest distribution while Osun-state had the lowest. 16.7% of the isolates harboured plasmids while 13.3% showed amplification using the SHVbla primer. ····· 1036125347

Impact & Assessment Of Bioaerosols In Hospital Environment

····· lezzter Preis 44.10€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Aeromicrobiology deals with the study of air borne microorganisms and viruses along with important particulate matter of air, especially smoke, dust and pesticides. Hospital is an important indoor environment responsible for the spread of airborne pathogens which later on is transmitted to other individuals viz., patients, hospital workers, visitors, etc. In turn it is carried over patients. Squames from the skin of persons may contain many pathogenic bacteria which are transmitted to patients. Hospital environment is responsible both for indoor and later outdoor community and represents a congenial situation where microorganisms and susceptible patients are together indoors. The estimation of the quantity and types of airborne microorganisms serves as an index for the cleanliness of the environment as well as a profile reveling human health and hospital-acquired infections. Evaluation of microbial count, types, and diversity in hospital indoor environments is of growing concern. Therefore, attempts must be made to check the spread of pathogens in the hospitals so that primary and secondary infections could be avoided. ····· 1036125304

Optimization for cellulase production by Aspergillus Niger

····· lezzter Preis 59.00€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Microorganisms bring about most of the cellulose degradation occurring in nature. In the present study, optimization for cellulase production by Aspergillus niger was investigated by using paddy straw as substrate.Cellulase enzyme was produced by the fungus extracellularly. Optimization of cellulase production was done by using various physical (Temperature, pH, Salinity and Incubation time) and chemical parameters (Carbon sources and nitrogen sources) which could influence the enzyme activity. Cellulase production was maximum at the temperature 20°C and minimum at 40ºC. The optimal pH for the cellulase production was observed maximum in 6.0 and minimum in 7.0. Cellulase production was maximum at 48 hours and minimum at 24 hours. Cellulase production was maximum with when fructose was used as a carbon source and minimum with sucrose. Cellulase production was maximum when Malt extract was used as a nitrogen source and minimum with yeast extract. The cellulase enzyme extract was partially purified and its protein fraction was stained with its molecular weight determination by using SDS-PAGE. It revealed two protein bands with the molecular weights of about 83 kD and 50kD. ····· 1036125273

Methicillin-resistant Staphylococcus aureus

····· lezzter Preis 26.91€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Staphylococcus aureus is widely recognized as the most significant nosocomial pathogen due to its virulence potential and because the species has acquired resistance to almost all current antibiotics. S. aureus is also an important cause of community-acquired sepsis worldwide. MRSA bacteraemia rates in the UK and internationally remain high and MRSA is endemic in many units despite widely publicized control regimens prompted by public concerns. Although it is known that in the UK 95% of nosocomial MRSA bacteraemia is caused by one of two major MRSA lineages, we know relatively little about their epidemiology. It has been difficult until recently to track the variants of these isolates that cause outbreaks of disease. High resolution multilocus DNA sequencing methods have been developed that can address this issue to provide for the first time a detailed analysis of S. aureus and MRSA genotypes. MRSA is changing from an infrequent cause of colonization to a persistent and costly cause of morbidity and mortality in hospitals and now increasingly in the community. The changes in MRSA epidemiology are impacting human health and will continue to do so. ····· 1036125266

Invitro Biofilm Production of Escherichia Coli O157:H7 Strain

····· lezzter Preis 44.10€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray
Biofilm is an important strategy used by some microorganisms for survival. The present study was carried out to optimize the invitro culture conditions required for biofilm formation of Escherichia coli O157:H7. Tryptic soya broth (TSB) was employed as the growth medium for biofilm production using chitin flakes as inert surface. The 0.08 percent with 0.3 percent w/v chitin flakes optimum concentration of TSB required for maximum biofilm production by Escherichia coli O157:H7. Biofilm cell population reached peak on day two, while planktonic cell population started decreasing after day one post inoculation. Decline of biofilm cells was gradual compared to planktonic cells. ····· 1036125051

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