für 71.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray -Amylase (EC 3.2.1.1) is a class of enzymes that is capable of digesting these glycosidic linkages found in starches , catalyzing the cleavage of the -1, 4-glucosidic linkages between glucose molecules in starch.With the beginning of new frontiers in biotechnology, the spectrum of - amylase application has expanded into many fields, such as clinical, medicinal, and analytical chemistries. - Amylase has many applications in the industrial process such as in the food, textiles and paper and Because of the importance of -amylase the current study`s aims are : Isolation and identification of Bacillus stearothermophilus from different soil samples, Production, purification ,characterization of some properties of thermolabile -amylase and molecular cloning of -amylase gene from Bacillus stearothermophilus in E. coli then investigate the possible expression of -amylase gene in the transformed cells. ····· 1036188608
für 71.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Plant cell wall structural proteins (CWPs) contribute to the basal defense against pathogens. Therefore, the prospect of developing disease resistant crop cultivars through genetic manipulation of CWPs is promising. Research presented here utilized a novel CW annotated Arabidopsis gene, the PELPK1 for functional characterization by using bioinformatics and computational analyses, and by applying various reverse genetics techniques, e.g., expression, mutational, and proteomic analyses. Specific techniques used are: (i) construction of several plant transformation vectors such as PELPK1-promoter::GUS reporter fusion, PELPK1-CDS::GFP translational fusion, RNA interference of PELPK1 + PELPK2, and constitutive over-expression of PELPK1, and development of transgenic lines harbouring the above vector constructs as well as sequence-indexed T-DNA insertions in PELPK1 gene, (ii) performing of various molecular techniques such as qRT-PCR, GUS staining, confocal laser-scanning microscopy, immunolabeling, TEM, 2D gel, LCMS/MS, and microtomy, and (iii) carrying out of genotypic and phenotypic analyses of the loss of function and gain of function mutant lines under various stress conditions ····· 1036188446
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Though the skill & technique of the surgeon are important for wound healing, so is the choice of suture materials. The choice of an appropriate suture for any wound closure will make a large contribution to the final functional & cosmetic result. An histological comparison of sutures is provided here to give readers a full understanding of wound healing when different types of suture materials are used. We sincerely hope that surgeons past, present & future will benefit from this research & thus treat their patients successfully. ····· 1036188237
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray This book has 8 chapters which include the introduction and role of computers in biotechnology as computational biology and bioinformatics. Due to its role in biological sciences and biotechnology, new name is given to this apllication as biocomputers. This application of computers in biotechnology as computational biology and bioinformatics is the phenomenonal and inevitable. This applied science is a excellent fusion of life sciences and computers. Hence, this application of computers is very helpul to solve some untold facts in biotechnology as in the field of forensic sciences, genetics, evolutionary biology,biochemistry, microbiology, etiopathogenesis of epidemics & genetic disorders, cell & molecular biology. I also express my profound sense of gratitude and sincere thanks to Mrs. Bhawna sharma(B.Sc. and M.C.A.[Master of Computer Application] from Maharashi Dayanand University, Rohtak, INDIA)for her encouragement and support during the writing of this book. She is also insipiration for me to complete the project of this book. Last but least, my indebted thanks to All Mighty and my loving parents, My Father,`Late Mr. Ram Kumar Sharma & my mother,`Mrs. Sushila Sharma. ····· 1036188102
für 71.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The horseshoe crabs or `King crabs are regarded as one of the most primitive animals existing in today`s world, dating back to the period of 300 millions years. Of only the four extant species, two are known from the eastern cost of the Indian region and because of their unique morphological similarities with the fossil, Mesolimulus walchi they are called `living fossils`. They play an important ecological role in the food web. A decline in its population will exert serious impact on other species. The biomedical industry produces a valuable substance known as ameboecyte lysate from the blood of horseshoe crabs. This substance is used to test a variety of biomedical products, vaccines and many other live saving drugs for the presence of endotoxins or any bacterial contamination. The present study is aimed at providing a foundation of vital information on its occurrence, habitat requirements, foods & feeding behavior, population dynamics with breeding activity and also neuro-endocrine function, which may lead to formulate an effective conservation cum-management strategy to preserve these threatened and endangered harmless creatures. ····· 1036188099
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Several lines of evidence have indicated that prion disorders are associated with an increase in cellular cholesterol content. Our findings highlight the existence of metabolic lipid alterations associated with prion infection. A higher content of cholesterol esters (CE) was accompanied by variations in the expression of ACAT-1 and Cav-1 in skin fibroblasts from scrapie-susceptible sheep. Increased levels and altered distribution of free cholesterol (FC), triglycerides, phospholipids and CEs were found in prion-infected N2a cells (ScN2a). Similar cholesterol alterations were observed in brains of Pravastatin-treated and untreated scrapie-infected mice: a high level of cholesteryl-arachidonate was associated with increased CEs in the brain while oral pravastatin administration caused a significant reduction of FC and an additional increase of CEs. In addition, the treatment with dual-drug combinations of a cholesterol ester modulator with a prion inhibitor had showed a synergistic anti-prion effect in ScN2a cells. These data support our hypothesis that certain pathways of cholesterol/cholesterol ester metabolism may represent suitable targets for prion disease therapy. ····· 1036188080
····· lezzter Preis 79.00€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Alopecia areata is a T-cell mediated autoimmune disease which mainly affects the anagen stage hair follicles, the most effective way of treating alopecia areata is the application of contact sensitizers which induces a mild chronic eczema (delayed type hypersensitivity). The curative effect of chronic eczema relies on the expansion of myeloid derived suppressor cells (MDSC) which can suppress the autoreactive T-cells and induces apoptosis of these via mitochondrial apoptotic pathway. Alternatively, a blockade of accessory molecules in T-cell activation could possibly be of therapeutic benefit. CD44 and CD49d being candidate accessory molecules since association of CD44 with CD49d plays an important role in homing of lymphocytes and blockade of these bystander pathways could be a therapeutic approach in alopecia areata. ····· 1036188047
für 61.20€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray This book investigates the use of different molecular techniques in detecting and enumerating toxic dinoflagellate species. Polymerase Chain Reaction (PCR) and catalyzed reporter deposition fluorescent in situ hybridization (CARD-FISH) have been used and optimized to detect and enumerate the target species. Epifluorescence microscopy and flow cytometry were used in this study. Epifluorescence microscope was shown to be superior for the quantification of the target species, although flow cytometer was demonstrated to be a promising technique for developing an automated detection system. ····· 1036188023
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Ganoderma lucidum (Leyss. ex Fr.) Karst. (Ling Zhi) (Aphyllophorales) (the family Polyporaceae) was first indexed in the Shen Nong s Materia Medica (206 BC 8 AD) as a longevity-promoting and tonic herb of the non-toxic superior class, and has been used in traditional Chinese medicine (TCM) for more than 2000 years to prevent and/or treat various human diseases such as hepatitis, chronic bronchitis, gastritis, tumor growth and immunological disorders. According to Fuzheng Guben , one of the major TCM therapeutic principles, Ganoderma lucidum (Gl) is capable of strengthening body resistance and improving constitutive homeostasis in patients (Lin, 2001). The name Ganoderma is derived from the Greek ganos/ `brightness, sheen`, hence `shining` and derma/ `skin`, while the specific epithet lucidum in Latin for `shining` and tsugae refers to being of the Hemlock (Tsuga). Another Japanese name is mannentake, meaning `10 000 year mushroom` (Liddell et al., 1980). The genus Ganoderma was named by Karsten in 1881. Members of the family Ganodermataceae were traditionally considered difficult to classify because of the lack of reliable morphological characteristics. ····· 1036188020
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The primary goal of bioinformatics is to increase the understanding of biological processes. What sets it apart from other approaches, however, is its focus on developing and applying computationally intensive techniques. Currently, different programming languages are being used in bioinformatics to develop innovative analysis tools ranging from sequence analysis to evolutionary studies.This book describes the development of sequence analysis software that provides sequence analysis tools required by researchers of different research fields. ····· 1036187924
für 53.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Chemicals such as industrial effluents induces some level of alterations in the naturally occurring chemical composition of aquatic phase which in turn alters the behavioral, biochemistry, and general physiology of aquatic fauna. Chemical additives effluent was analyzed to determine its physicochemical parameters. The fish, Clarias gariepinus was exposed to lethal concentrations of the effluent for 96 hours and the (96-h LC50) value for the acute toxicity was found to be 0.30mg/L. Mortality of exposed fishes increased as the concentrations of the effluent increases. The impact of long term exposure to the effluent was also evaluated through changes of biochemical and haematological parameters as well as the assessment of heavy metal bioaccumulation in selected biomarkers. It was concluded that fish can bioaccumulate heavy metals from polluted environments. The chemical additives effluent had some degree of negative impact on the biochemistry, haematology and metal bioaccumulation pattern of Clarias gariepinus. This finding should be useful to ecotoxicologists, aquatic resources managers, laboratory scientists, researchers and students at various levels of higher learning. ····· 1036187900
für 61.20€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The hazardous agricultural chemicals lead to severe problems of human health and environment. A switch over from the use of synthetic fertilizers and pesticides to organic farming is now imminent. Earthworms have been long recognized to contribute in the decomposition process and in soil fertility enhancement. Earthworms harbour a multitude of microorganisms in their gut. In the present work four earthworm species viz. Metaphire posthuma, Lampito mauritii, Eisenia foetida and Perionyx excavatus were studied to find the most predominant gut bacteria species. Besides aiding in composting, these bacteria were also found to increase the yield of the common Indian vegetables. One of these bacteria has significant role on speedier germination of seeds. The present work indicates that these earthworm gut bacteria can be efficiently utilized in various organic farming strategies and have a potential importance to be utilized as biofertilizer. Here lies the significance of the work because earthworm gut bacteria had not been previously studied to find their effect on plant growth. ····· 1036187840
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The influence of different management practices (insecticide, plant extract, homeo chemical & biological control) on the mealybug (Phenacoccus solenopsis) infestation was studied to evaluate their potential as a management strategy. Five treatments viz. chemical control (Profenofos 50 EC), plant product (Neemosal 0.5% EC), homeo-chemical (Fierce), biological control agent (Chrysoperla carnea) and a control plot were tested in three replicates. Profenofos showed the best control against cotton mealybug population. Neemosal and Fierce did not notably lower the mealy bug population while C. carnea failed to produce any result. It is suggested that neem based insecticide (Neemosal) and homeo chemical (Fierce) can be applied during initial or low mealybug infestation, however chemical control (profenofos) should remain as the last option during heavy infestations. ····· 1036187821
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Reductional nature of meiosis is responsible to maintain the ploidy in eukaryotes. In sexual reproduction, Meiotic division produces gametes of half ploidy and fertilization restores original chromosome number. In the meiosis sister chromatids are monoorientated which leads to separation of homologous chromosomes. This is followed by separation of sister chromatid in meiosis II because of biorientation of sister chromatids. Comparison of meiosis with mitosis reveals that monoorientation of sister chromatids is the main reason for halving the ploidy. According to the present hypothesis, Monopolin complex, A four subunit complex, directs monopolar attachment of sister chromatids. Present study is done to reveal the nature and function of this complex. Cell cycle arrest mutants were created and verified to maximize the number of cells at particular stage by deleting or shuffling the promoter of a particular gene. Then, Csm1 subunit of monopolin complex was affinity tagged and verified in arrested cells. As future prospects of this work affinity purification of Csm1 can be done. Comparison of protein profiles obtained will help to reveal the functional properties of monopolin complex. ····· 1036187805
für 71.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray One of the most pressing objectives of medical research today is the development of approaches to restore the function of damaged tissues. An important goal for this work is the isolation of stem cell populations to replace missing or nonfunctioning cells. Because problems of immune rejection a desirable strategy is to convert differentiated cells from patients into immature tailored stem cell populations. Reprogramming differentiated cells towards pluripotency can be achieved by different experimental strategies including the forced expression of specific inducers and nuclear transfer. While these offer unparalleled opportunities to generate stem cells and advance disease modelling, the relatively low levels of successful reprogramming achieved makes a direct analysis of the molecular events associated with productive reprogramming very challenging. The generation of transient heterokaryons between human differentiated cells (such as lymphocytes) and mouse pluripotent stem cell lines developed by Carlos Filipe R. L. Pereira provides an alternative approach to study early events during reprogramming. ····· 1036187802
für 71.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Blood pressure variability (BPV) is one of the recognized risk factor for different types of cardiovascular diseases. In recent times there has been an alarming increase in the incidence of hypertension and cardiovascular diseases (CVD) in India. It is presumed that the phenomenon could be attributed to concomitant prevalence of abnormality in the blood pressure variability (BPV). We aspire that the results obtained and documented in this work will provide the scientific community with the novel data on BPV in human population of India that has never been studied earlier. In addition, a comparative account of BPV among healthy and hypertensive subjects may contribute significantly to the existing knowledge on the discipline. The outcome of the study might help in: (a) establishing the standard values of blood pressure and heart rate with special reference to gender and age, (b) assessing the relative role of predictors of hypertension, and (c) delineating plans to curb various cardiovascular diseases in India. ····· 1036187771
für 59.00€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Rapid world population increasing results in a rising demand for protein for both human and animal consumption. This demand is certainly become serious with overexploitation of the sea and the use of most of the available arable land as the population rapid growth continues. We are challenged with finding of new protein sources that will not require land space or costly and tedious means of production. Microorganisms could serve an ideal solution to this problem. The single cell of a microorganism is itself a perfect protein factory. Hazardous pollution occurs from agricultural and industrial wastes, e.g. whey as a serious pollutant, has stimulated interest in converting waste materials into commercially valuable products, especially single cell protein (SCP). In competing for limited resources, microorganism evolves many strategies for selective advantages one of these is the secretion of toxic compounds that kill or arrest the growth of other species, i.e. yeast killer toxins, which could be invested in many applied fields. The present work was designed to deal with these issues through bio-conversion of agro-industerial wastes into SCP using genetically modified killer yeast ····· 1036187730
für 53.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Evolutionary conservation of protein interaction properties has been shown to be a valuable indication for functional importance.Rodent orthologs are more conserved in human and the human disease genes are functional in rat genome. Some are inherited disease gene are highly conserved in rat. The investigation of evolutionary rates among large sets of genes has become feasible with the availability of the genome sequences of human, rat & mouse. Evolutionary conservation of protein composition and biochemical properties is usually a valuable indication for the cellular importance of a specific protein complex. In this study we analyzed whether association rates of protein in 5 major biochemical pathways of autoimmune disorders are conserved during evolution, because evolutionary conservation of protein biochemical properties is usually a valuable indication of its importance. ····· 1036187657
für 49.00€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray In recent years science and technology have made big steps, allowing us to greatly improve knowledge in basically every aspect of biology, culminating in the mapping of the human genome. However, although the brain is considered our most vital organ, the essence of personality and memory, much about the human brain still remains a mystery. Morphological analyses seemed to support the idea that senescence was accompanied by neuronal loss, particularly evident in the cortical and hippocampal structures. The work embodied in this book was carried out to find out whether administration of neuroprotective and anti-aging compound dehydroepiendrosterone (DHEA) at different ages of the animals prevents the delay of biochemical, molecular, structural and physiological alterations know to the neurological disease associated with aging. Based on study, the DHEA beneficial effects seemed to arise from their antioxidative, antiobesity, antilipofuscin and antilipidperoxidative actions. ····· 1036187572
für 61.20€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Mathematical models for revealing the dynamics and interaction properties inside biological systems play an important role in computational systems biology. This work is motivated by the current difficulties in identifying practical bio-system models. In the field of systems biology, available data are often noisy, sparse and expensive to collect. Therefore, system identification is a challenging problem. A major task of identifying a model, as described in terms of non-linear ordinary differential equations (ODEs) with indeterminate parameters, can be formulated into an optimization problem. It is a reverse engineering exercise to reconstruct the system model via various numerical tools like constraint-mixed-optimization algorithms and approximations. Due to sensitivity issues, in many cases, even the simulated output data, as generated by the identified model with a set of estimated parameters, fit very well with the measured data, it is still important to infer how well these model parameters being determined which is essential for the investigation of model construction. For this reason, the identifiability issues, which is an important practical issue, is also treated. ····· 1036187553
für 61.20€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The book deals with chitanse production by actinomycetes by solid state fermentation. Actinomycetes have been widely exploited group of microorganisms in the production of secondary metabolites and enzymes of commercial importance. Chitinases have received attention as they have been widely used as biocontrol agents for suppression of pathogenic fungi, mosquito control, mycolytic enzyme preparation, fungal protoplast technology, preparation of chito-oligosaccharides, glucosamine, and, cytochemical localization of chitin/chitosan using chitinase-chitosanase-gold complexes, production of single-cell protein, estimation of fungal biomass, morphogenesis, medical application, control of plant pathogenic fungi and degradation of fish wastes, etc. Solid state fermentation (SSF) holds tremendous potential for the production of chitinases specially in those processes where the crude fermented products may be used directly as the enzyme source. SSF could be the system of choice for microbial enrichment of agriculture residues and the production of enzymes and other extracellular metabolites. The book will be of great value for researchers as well as for people of industry. ····· 1036187536
für 53.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Molecular techniques are now a valuable tool for achieving advances in genome research and generating considerable interest in predicting hybrid performance. Molecular markers are of great value in genetic research and partially in breeding programs since they reflect the genetic variation among individuals. Various PCR-based marker techniques have recently been successfully introduced in the fingerprinting of plant genomes and in genetic diversity studies. Among them random amplified polymorphic DNA (RAPD) analysis which is relatively simple, rapid and cost effective. It is hoped that the present study may help maize breeders to produce new hybrid varieties having higher yielding potential. ····· 1036187439
für 61.20€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Hyperlipidemia is defined as a condition in which there is an excessive amount of body fat. This is one of the most commonest diseases all over the world. The incidence of hyperlipidemia has dramatically increased and has become epidemic in the western as well as eastern world. The etiology of hyperlipidemia is multifactorial, with genetic, environmental, socioeconomic and behavioral or psychological influences, with an increase in the related morbidity and mortality. This study was undertaken to investigate the potential role of Caulerpa prolifera and Sargassum subrepandum methanolic extract in management of hyperlipidemia and its complications in adult female rats. These results indicated that C.prolifera and S.subrepandum algal extracts play a vital role in ameliorating hyperlipidemia and its complications particularly oxidative stress and inflammation. These findings may provide new concept for the development of effective marine natural therapy for hyperlipidemia and its associated serious complications. ····· 1036187403
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The book in hand can furnish baseline information about the detection of various pathogens using different laboratory techniques i.e., Basic Laboratory Procedures Sterilization and Disinfection, Computer in Laboratory, Indenting and storage of Reagents, Hazards in Laboratory, Collection, Transport, Storage and Disposal of Specimens. General Clinical Pathology Urine Examination. Hematology Hemopoesi. Anemia s. Estimation of Hemoglobin, Red Cells Indices, Erythrocyte Sedimentation Rate, Preparation and Staining of Blood Film, Total Leucocytes Count, Platelet Count, Reticulocyte Count. This can be an asset for the medical students, laboratory technicians and allied stalk holders. ····· 1036187264· 1 · ::::: · 20 ·· 21 ·· 22 ·· 23 ·· 24 ·· 25 ·· 26 · ::::: · 36 ·
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