für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The striking similarities between the innate defenses of vertebrates and invertebrates as well as the amenability of Caenorhabditis elegans for genetic analysis have made this free-living ground nematode a popular model system in the study of bacterial pathogenesis. Although genetic studies have brought new insights, showing the inducibility and pathogen-specificity of the immune response, there is still much to be discovered about the exact mechanisms underlying resistance to infection. In this study a different angle was adopted to study host pathogen interactions in C. elegans. Given the dynamic nature of an immune response, the proteome of C. elegans was investigated at four different time-points (24, 48, 72, 96 h) after infection with S. typhi, S. typhi Ty21a, C. sakazakii and E. coli OP50 using SDS PAGE as a preliminary step to detect variation in the whole protein content. ····· 1036198273
für 61.20€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The quality of water is now the concern of experts in all countries of the world. The River Nile constitutes very important aquatic and wetland ecosystems and it supplies about 97% of Egypt s water reserves. Man frequently destroys the stream s capacity for natural self purification through excessive additions of domestic sewage and industrial wastes. Therefore, a wide variety of pathogens may be transmitted by faecally polluted water. Densely populated areas with inadequate sanitation and sewerage facilities served as a major source of pollution of surface water supplies and Rivers. Different genera of pathogenic bacteria were detected in River Nile water, Egypt. Waterborne bacterial pathogens are causative agents for many human diseases, and their presence in water bodies poses a potential threat to the human population. Because of the risk of public outbreaks of disease, it is of utmost importance that communities be aware of potential risks associated with water contact, including the presence of frankly pathogenic bacteria. ····· 1036192525
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Diabetes mellitus, one of the most common endocrine metabolic disorders, has a significant impact on the health, quality of life, and life expectancy of patients as well as on the health care system. Appropriate management might reduce the burden of common infections. The aim of this study in general practice was to identify and determine the microbiological profile and antibiotic susceptibility patterns of organisms isolated from the diabetic patients. 50 patients presented with one or more infections and the pus fluid was collected from these patients. Different types of pathogenic bacteria have been isolated from the pus samples of infected areas in the patients with diabetes. The type of isolates includes pathogenic group of bacteria, anaerobic organisms and other anaerobic non-spore forming Gram positive and Gram negative rods. Among the 50 samples collected, 25 contain, -haemolytic Staphylococci, 5 contain non-haemolytic Streptococci and 20 samples contain both, -haemolytic as well as non-haemolytic Staphylococci. The isolated organisms were also confirmed by depend upon their biochemical activities. ····· 1036192464
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray This book presents a critical understanding of two major types of widespread forms of infectious diseases, caused by Mycobacterium tuberculosis and Human immunodeficiency virus (HIV). The social, economic, cultural and political determinants affecting the health and well being of populations around the world, specifically Sub-Saharan Africa, in relation to tuberculosis and HIV/AIDS are addressed. In this book, the following has been detailed: An account of tuberculosis and HIV co-infection in Sub-Saharan Africa (Causative agent, condition symptoms, history and epidemiology of tuberculosis setting profile, policy and public health priorities treatment or intervention options and management of TB/HIV co-infection) promotion of the infant formula use to prevent HIV transmission from mother to child in Western Africa and assessment of the use of condom to prevent HIV transmission within the East African community. ····· 1036192413
für 53.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Candida tropicalis is a common species related to nosocomial infections, namely candidemia and candiduria. Several virulence factors seem to be responsible for C. tropicalis infections, which present high potential for dissemination and mortality. Adhesion to surfaces (medical devices and host cells) and biofilm formation, are considered important factors that contribute to the development of candidosis. Further, adhesion and invasion of host cells by C. tropicalis is considered the first step to initiate systemic infections. Once adhered to epithelium, C. tropicalis are able to secrete hydrolytic enzymes that cause damage in host cells membrane integrity, leading to dysfunction or disruption of host structures. Thus, the main aim of this work was to characterize the virulence factors of C. tropicalis as well as to evaluate adhesion to biotic and abiotic surfaces, biofilm formation, expression of hydrolytic enzymes and antifungal susceptibility of C. tropicalis clinical isolates from urine and blood cultures and from central venous catheters. ····· 1036192358
für 53.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Marine environments were investigated and 34 actinomycetes were isolated: 21 Micromonospora strains, 10 Streptomyces, and 3 Pseudonocardia. Potentially, all 21 Micromonospora strains are novel, as revealed by an original identification scheme developed to assess quickly and easily the novelty of newly isolated environmental Micromonospora strains. Standardized media for testing physiological characters of Micromonospora were developed, and additional physiological characteristics of 15 of the validly published members of Micromonospora are described. Micromonospora isolates grew under anaerobic conditions. 8 new Micromonospora species are proposed. The antibiotic biosynthetic capability of strains was assessed by PCR targeted amplification of antibiotic biosynthetic and resistance genes (oxyB, ARO-PKS, ANSA, grm, fmr). An aminoglycoside antibiotic susceptibility plate assay and the grm and fmr PCR primers revealed the presence of aminoglycoside resistance genes in the genome of Micromonospora and suggested that these strains are capable of synthesizing aminoglycoside-type antibiotics. ····· 1036192317
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Alkaline protease producing Baciilus spp. were isolated from natural habitats of Lahore. Thirty eight isolates exhibited the prominent zones of clearance on skim milk agar medium at pH 10. These isolates which showed best proteolytic activity were then identified. Twenty two (60 %) of the alkalophilic isolates belonged to the genus Bacillus. Maximum enzyme activity (191.0U/ ml) was found in strain isolated from slaughterhouse (coded as MPPL1) at pH 10 and temperature 37°C. Maximum activity was obtained at pH 10, 140 rpm, at 37°C after 24 hours of incubation. The results indicated that protease producing acillus strain coded as MPPL1 is active at normal ranges of temperature and high pH. Alkaline protease producing conditions i.e, culture media, pH, temperature, agitation speed, incubation period were optimized at shake flask level for maximum enzyme activity and these conditions were applied at bioreactor for submerged fermentation in 1 litre jar bioreactor, an increase of nearly 80% proteolytic activity was observed after obtaining crude enzyme by centrifugation the fermented broth at 10000rpm for 10 minutes at 4°C. ····· 1036192212
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Identification and conservation of microorganism s biodiversity is very important for the service of mankind. Microorganisms (Fungi and Bacteria) are conserved in living states in the form of cultures. For proper growth requirements, different techniques are essential for culturing. Organisms causing disease are either present on the surface of leaf, branch, fruit, stem and roots or inside the plant tissues of these parts or on seed or inside the seed tissues. For efficient plant disease identification, it is essential that the plant pathologist should be well aware in the identification of pathogens and has good command and knowledge of identification and characters of pathogens. Before starting the isolation of any pathogen from a disease specimen of any plant, it is necessary to know the basic techniques for isolation of pathogen. Scientists isolating microorganisms may have a variety of objectives. They may be involved in environmental, taxonomic, agricultural or biochemical research, or be interested in screening for novel products that may have commercial value and for the purposes of describing new taxa and to act as essential reference standards for future study. ····· 1036192130
····· lezzter Preis 61.20€ ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Helicobacter pylori is a gram-negative bacterium that forms the etiology of chronic gastritis and peptic ulcer disease. Infection with H. pylori can be effectively treated by the combination of a proton pump inhibitor in addition Clarithromycin and amoxicillin. Emergence of Clarithromycin resistant strains of was not well studied in Sudan. Clarithromycin is a macrolide that binds to the 23S rRNA components of the bacterial ribosome. Resistant strains emerge due to failure of such binding because of modification of the target site by point mutations in the peptidyl transferase region of domain V of the 23S rRNA. H. pylori contain two copies of the 23S rRNA gene8. Several points mutations have been reported that are associated with macrolide resistance, but the most common is A-G transitions at position 2143 (A2143G). ····· 1036192076
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray A prospective cross sectional study was carried out on women who attended the family planning and gynaecologic unit of the Dschang District Hospital and other healthcare centres. This study determined the types, risk factors, pathogens of sexually transmitted diseases and other genital infections and the in vitro susceptibility profile of the most commonly isolated bacterial pathogen to ten antibiotics by a disc diffusion method on Mueller Hinton agar. Out of the 1820 women encountered in this study, 1024 ( 56.3%)women were presenting symptoms of sexually transmitted diseases and other genital infections while 796 (43,7%) were asymptomatic for these infections. Major determinants of genital infections were unprotected sexual intercourse, promiscuity, extra marital sexual practices, multiple sexuality, poverty, poor personal and environmental hygiene, frequent wearing of tight fitting clothes and synthetic underwear, the use of medicated soaps to clean the genitalia, and immunosuppression ( pregnancy and AIDS). Vancomycin (100%), gentamycin (91.7%), augmentin (87.7%), ciprofloxacin (81.3%), and streptomycin (81.0%) had high in-vitro efficacy against S. aureus strains. ····· 1036192057
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Green synthesis of nanomaterials is gaining importance day by day especially concerned due to environmental reasons. An array of physical, chemical and biological methods have been used to synthesize nanomaterials. In order to synthesize noble metal nanoparticles of particular shape and size, specific methodologies have been formulated. Physco-chemical and other reduction techniques have been used successfully to produce nanoparticles, but they remain expensive and involve the use of hazardous chemicals. Therefore, there is a growing concern to develop environment-friendly and sustainable methods. Since the synthesis of nanoparticles of different compositions, sizes, shapes and controlled dispersity is an important aspect of nanotechnology new cost effective procedures are being developed. Microbial synthesis of nanoparticles is a green chemistry approach that interconnects nanotechnology and microbial biotechnology. ····· 1036192028
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Present study aimed at the detection of microbial contamination present in commercially available and freshly prepared orange juice samples collected from different localities of Lahore, Pakistan. Results showed the presence of Enterococcus spp., Streptococcus spp., Micrococcus spp., Staphylococcus spp. and Bacillus spp. in commercially available orange juices which clearly demonstrates the unhygienic conditions of processing plants. In most localities, the freshly squeezed orange juices were hygienically very poor as bacterial loads on the whole were abnormally high. Based on the presence of Enterococcus spp., Streptococcus spp., Micrococcus spp., Staphylococcus spp., Bacillus spp., Erwinia spp., Salmonella spp., Klebsiella spp., Coliforms, Pseudomonas spp., Vibrio spp. and Leuconostoc spp. in fresh orange juices, it is concluded that juices in certain areas inside the city are highly impacted and unfit for human consumption. It is agreed that such type of contamination is the result of bad quality water used for dilution of juices, unhygienic surroundings and location of stalls by the side of a busy road with vehicular traffic or by the side of waste disposal and overcrowding. ····· 1036191721
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Actinomycetes spp. can be a good source of antibacterial metabolites. The metabolites extracted here can be used as a full proof drug after proper pharmacological evaluation. Future work of present investigation includes identification of the isolate, Phytochemical screening of the antibacterial metabolite, evaluation of antifungal properties, determination of MIC (minimum inhibitory concentration). ····· 1036191665
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Biodegradation of paranitrophenol (PNP) was carried out using Pseudomonas pseudomallei capable for PNP degradation. For enhanced biodegradation this strain was subjected to UV ray, chemical and gamma ray mutagenesis. UV and chemical mutagenesis was not fruitful as no PNP hyper degrading bacterial strain was obtained. Gamma ray mutagenesis was positive for PNP degradation and hyper degrading bacterial strain was obtained. Both parent and mutant in presence of glucose, efficiently degraded PNP. When concentration of glucose increased from 50 to 100 ppm then 50 ppm PNP was degraded within 12 hours by mutant and in 20 hours by parent. So glucose had positive effect on PNP degradation and resulted in enhanced PNP degradation. Effect of phenol was also checked on PNP degradation. The result showed that immobilization of the bacterial culture was best practice for PNP degradation. Similarly addition of glucose helped enhanced PNP degradation and ammonium sulphate provided good nitrogen source. After this toxicity of treated and untreated PNP was also checked by exposing to fish, that showed that after degradation of PNP no toxic metabolites were produced. ····· 1036191507
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Hepatitis C virus (HCV) infection is one of the most important Flavivirida infections with significant clinical problems throughout the world in humans. Six major Hepatitis C virus (HCV) genotypes and hundreds of subtypes have been identified globally. All these genotypes are generally studied for epidemiology, their vaccine development and clinical management. This thesis comments the frequency distribution of various HCV genotypes circulate in different areas/districts of Khyber Pakhtoonkhaw Province of Pakistan. Out of the tested samples, genotype specific PCR fragments were observed in 299 (74.82%) patient serum samples. The distribution of genotypes of the typeable samples was as follows: 3 patients (0.72%) each were infected with genotype 1a and genotype 1b 240 patients (80.26%) of genotype 3a 25 patients (6.00%) genotype 3b and 28 patients (6.73%) were observed as with mixed genotypic infection. Sums of 116 serum samples (27.88%) were still found untypeable by the used molecular genotyping system. In conclusion, HCV genotypes 1a, 1b, 3a and 3b are distributed in various parts of KPK among which the genotype 3a is the most frequent genotype. ····· 1036191500
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Hemorrhagic septicemia has been an acute and fatal disease among cattle and buffaloes caused by Pasteurella multocida. The prevalence of this disease is 49% in animals of Pakistan. The present study was to evaluate the prevalence, multidrug resistance and the molecular heterogeneity within clinical strains of P. multocida collected from different cities of Pakistan. Tissue samples of lung and spleen of dead animals were used for diagnostics. Rapid plate screening test for Hyaluronidase of clinical Isolates showing clear and white zones proved that given isolate is pathogenic. Amongst one hundred and ninety nine total isolates, only one hundred and one isolates were found to be virulent as clinical isolate of P. multocida. Isolates were confirmed through amplification (PCR) of specie specific and Hemorrhagic septicemia type B causing test. The primer pair of specie specific test showed a band of 300bp in all subspecies of P.multocida whereas the primer pair of HS type B causing test showed a band of 460bp in all HS-causing serotype of P.multocida. The high prevalence was found only in Karachi. Whole cell and envelope protein profile of the isolates on SDS-PAGE showed presence of ····· 1036191468
für 55.71€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Enzyme production from lignocellulosic biomass is sustainable owing to their ubiquitious nature and non-competitivness with food crops. Agrowastes accumulate in envonment in substantial amounts as pollutants, and primarily comprise of cellulose and hemicellulose that can be saccharified by cellulases to produce fermentable sugars yielding bioethanol. However, cellulase production cost is major impediment in bioenergy production scenario. Saccharum spontaneum is an abundantly found agrowaste with high cellulose content which can be employed for cellulase production through biological route. In present investigation, cellulase production was significantly elevated through substrate delignification and process optimization by employment of fungal strain Trichoderma viride. Therefore, cost effective cellulase production can be achieved by exploiting cheaper substrates, effective pretreatment and process enhancement strategies for economical fermentation. ····· 1036191447
für 71.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Since ancient times microorganisms have proven themselves a protective warrior to fight against many problems in the favor of humankind and therefore their potential applications are expected to increase significantly in various area. Microorganisms are known to detoxify or magnify the toxicity of allelochemicals after their entry into soil, and thus influence allelopathic activities. There could be more than one mechanisms of action of such microbes affecting the fate of allelochemicals in soil. Therefore future allelopathy research should be designed to understand the interaction of allelochemicals with soil microbes. However, their significance in influencing allelopathic activity is largely not investigated in bioassays for allelopathy. In this book, therefore, an attempt has been made to focus mainly on this aspect of allelopathy where phytoxicity of invasive weed can be minimized by using beneficial rhizobacteria without having any harmful effects on non-target plants, soil and ecosystem. An approach has been made to cover all possible aspects of soil microbial ecology in allelopathic interactions which can provide a baseline for futuristic studies. ····· 1036191372
für 71.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray The leather manufacturing industries generate 350 kg of non-tanned solid wastes that include proteinaceous animal fleshing (ANFL). The recovery of value added products such as enzymes from the solid wastes has been largely a neglected field. Reports on the utilization of ANFL as the substrate for the production of acid protease are very scanty or perhaps nil. The present investigation was focused on the production of acid protease from animal fleshing (ANFL) as the substrate using isoloated acidophiles. The enzyme was purified and immobilized in mesoporous activated carbon. The immobilized acid protease was used for the degradation of proteins in aqueous solution. ····· 1036191343
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray Faisalabad is known as a city of textile not only in Pakistan but also all over the world. Several textile processing units are in operation in the surroundings of the city, which are releasing a substantial amount of azo dyes into the wastewater streams in the surroundings of these textile units.The discharge of these contaminants is considered to be a potential threat to the environment because of their hazardous nature. Thus, this study was undertaken to isolate the bacteria possessing high potential to degrade azo dyes from the wastewater discharged by the textile units. A total of 150 bacterial strains were isolated from these wastewater samples using minimal salt medium spiked with Reactive Blue ZBLN azo dye. Results revealed that some bacterial strains were able to decolorize 8 different dyes almost within 6 hours. Thus, the isolated bacterial strains have the potential to remove a variety of dyes from the liquid culture, which could be used to develop a biotreatment strategy for cleanup of the industrial wastewater prior to their discharge into wastewater streams of the surrounding area. ····· 1036191291
für 44.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray La zona central de la Patagonia Argentina se encuentra sometida a explotación petrolera y sus suelos se caracterizan por ser deficientes en nitrógeno. Los hidrocarburos contaminantes de un suelo pueden ser remediados utilizando métodos biológicos, para lo que es muy importante el nitrógeno biodisponible. Dicho nutriente es esencial para una adecuada formación de biomasa microbiana. Los procesos biológicos se pueden realizar en deficiencia de nitrógeno, pero con una menor eficiencia. En este trabajo, se estudió el efecto de la presencia y ausencia de nitrógeno sobre la degradación de hidrocarburos alifáticos y aromáticos, por medio de un estudio en microcosmos y el aislamiento y caracterización de cepas bacterianas a partir del suelo contaminado, capaces de biodegradar hidrocarburos en presencia de concentraciones deficientes de nitrógeno. A tal efecto se planteo como objetivo general del trabajo estudiar las capacidades de biodegradación por parte de comunidades y cepas aisladas que crecen a expensas de hidrocarburos y en déficit de nitrógeno y su posible uso como inoculantes para la biodegradación de hidrocarburos en suelos esqueléticos. ····· 1036191188
für 35.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray En Candida albicans la vía del AMPc/PKA está implicada en la transición levadura micelio. Los genes que codifican para las subunidades catalíticas de la PKA, TPK1 y TPK2, y el único gen que codifica para la subunidad regulatoria, BCY1 han sido caracterizados. Si bien resultados nuestros y de otros laboratorios han establecido funciones positivas para ambas isoformas de las Tpks en el crecimiento y en la filamentación, se han descripto algunas diferencias a nivel funcional dependiendo las condiciones de cultivo. Sin embargo al momento de comenzar este trabajo no se habían descripto roles específicos para las subunidades de la PKA en otros procesos celulares. La posibilidad de trabajar con un amplio conjunto generado de mutantes de PKA carentes de uno o ambos alelos de TPK1 y TPK2 en distintos céspedes de regulación nos permitió encarar el estudio de los roles de las subunidades catalíticas de la PKA en diferentes procesos metabólicos y/o fisiológicos como la respuesta celular frente a diferentes tipos de estrés, la participación de las Tpks en el crecimiento pseudohifal, la adhesión a sustratos abióticos y la formación biofilms, una capacidad vinculada a su virulencia. ····· 1036191143
für 26.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray La conservación de nuestro ecosistema depende de nuestra responsabilidad y la concientización de nuestros hijos, la biotecnología nos ayuda a resolver problemas, facilitar nuestra vida y ayudar a mejorar nuestro ambiente. La posibilidad de realizar estudios científicos para ayudar a disminuir y eliminar la contaminación por metales pesados provocada por nuestras actividades económicas, como la industria minera por ejemplo, se puede resolver utilizando microorganismos esta posibilidad es mas económica. La Bioremoción de metales pesados con la ayuda de bacterias resistente a metales pesados para recuperar sistemas acuíferos soluciona los problemas de contaminación provocadas por la industria minera, esto hace que nuestra preocupación por la conservación del ambiente aumente. Este trabajo se basa en motivar y tomar conciencia para recuperar el medio en que vivimos pensando en la conservación del ambiente y el manejo de la biotecnología como una ayuda en nuestra vida cotidiana. ····· 1036190749
für 26.10€ kaufen ···· Rheinberg-Buch.de - Bücher, eBooks, DVD & Blu-ray El género Candida spp hace parte de un grupo de micosis que se pueden encontrar dentro de la biota normal de los seres humanos en sus diferentes fases dependiendo las condiciones del medio ambiente que las rodea, pero que frente a un cambio hostil en el huésped toman el papel de agentes oportunistas que se van a caracterizar por afectar a los pacientes con ciertos factores predisponentes. La aspergilosis define todos los síndromes clínicos producidos por el hongo filamentoso denominado Aspergillus, éste hongo abarca un gran espectro de enfermedades cuyo desarrollo se relaciona con las características inmunológicas del hospedero por lo cual se ha planteado que la aspergilosis es producida en su mayoría en presencia de desorden inmunológico de base. La aspergilosis afecta varios órganos, principalmente el SNC, seguido por los pulmones. La pitiriasis versicolor (PV) es una micosis superficial, ocasionada por hongos dimorfo lipofílico caracterizados en el género Malassezia spp. Este libro hace énfasis en la etiología, inmunología, diagnóstico y tratamiento de estas micosis desde el punto de vista de la inmunofisiopatogenia de las enfermedades infecciosas. ····· 1036190692· 1 · ::::: · 9 ·· 10 ·· 11 ·· 12 ·· 13 ·· 14 ·· 15 · ::::: · 24 ·
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